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Copyright © Médecins Sans Frontières
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Showing 1 - 20 of 20 Items
Showing 1 - 20 of 20 Items
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Journal Article
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Research

Evaluation of OMNIgene® SPUTUM and ethanol reagent for preservation of sputum prior to Xpert and culture testing in Uganda

Ardizzoni E, Orikiriza P, Ssuuna C, Nyehangane D, Gumsboga M,  et al.
2019-10-16 • Journal of Clinical Microbiology
2019-10-16 • Journal of Clinical Microbiology
Background: Xpert MTB/RIF (Xpert) and culture are the most reliable methods for tuberculosis diagnosis but are still poorly accessible in many low resource countries. We aimed to assess ...
Journal Article
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Research

Cetyl-pyridinium chloride is useful for isolation of Mycobacterium tuberculosis from sputa subjected to long-term storage

Pardini M, Varaine FFV, Iona E, Arzumanian E, Checchi F,  et al.
2005-01-01 • Journal of Clinical Microbiology
2005-01-01 • Journal of Clinical Microbiology
Recovery of Mycobacterium tuberculosis from sputa treated with cetyl-pyridinium chloride (CPC) and stored for 20 +/- 9 days was significantly higher than that from sputa that were untrea...
Journal Article
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Research

Diagnostic accuracy of the small membrane filtration method for diagnosis of pulmonary tuberculosis in a high HIV prevalence setting

Boum Y II, Kim S, Orikiriza P, Acuña-Villaorduña C, Vinhas S,  et al.
2016-03-30 • Journal of Clinical Microbiology
2016-03-30 • Journal of Clinical Microbiology
Sputum acid-fast bacilli (AFB) smear microscopy has suboptimal sensitivity but remains the most commonly used laboratory test to diagnose pulmonary tuberculosis (TB). We prospectively ev...
Journal Article
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Research

Outbreak of hepatitis E virus infection in Darfur, Sudan: effectiveness of real-time reverse transcription-PCR analysis of dried blood spots

Merens A, Guerin PJ, Guthmann JP, Nicand E
2009-06-01 • Journal of Clinical Microbiology
2009-06-01 • Journal of Clinical Microbiology
Biological samples collected in refugee camps during an outbreak of hepatitis E were used to compare the accuracy of hepatitis E virus RNA amplification by real-time reverse transcriptio...
Journal Article
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Research

Improving the specificity of Plasmodium falciparum malaria diagnosis in high-transmission settings with a two-step rapid diagnostic test and microscopy algorithm

Murungi M, Fulton T, Reyes R, Matte M, Ntaro M,  et al.
2017-05-01 • Journal of Clinical Microbiology
2017-05-01 • Journal of Clinical Microbiology
Poor specificity may negatively impact rapid diagnostic test (RDT)-based diagnostic strategies for malaria. We performed real-time PCR on a subset of subjects who had undergone diagnosti...
Journal Article
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Research

Rapid detection of Mycobacterium tuberculosis resistance to second-line drugs by use of the manual mycobacterium growth indicator tube system

Martin AIC, Von Groll A, Fissette K, Palomino JC, Varaine FFV,  et al.
2008-12-01 • Journal of Clinical Microbiology
2008-12-01 • Journal of Clinical Microbiology
The objective of this study was to evaluate the manual mycobacterium growth indicator tube (MGIT) system for the testing of Mycobacterium tuberculosis susceptibility to second-line drugs...
Journal Article
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Research

Field evaluation of a simple fluorescence method for detection of viable Mycobacterium tuberculosis in sputum specimens during treatment follow-up

Schramm B, Hewison CCH, Bonte L, Jones W, Camelique O,  et al.
2012-05-30 • Journal of Clinical Microbiology
2012-05-30 • Journal of Clinical Microbiology
Simple tuberculosis (TB) treatment monitoring tools are needed. We assessed the performance of fluorescein-diacetate (FDA) smear microscopy for detection of viable Mycobacterium tubercul...
Journal Article
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Research

Lowenstein-Jensen Selective Medium for Reducing Contamination in Mycobacterium tuberculosis Culture

Kassaza K, Orikiriza P, Llosa AE, Bazira J, Nyehangane D,  et al.
2014-07-25 • Journal of Clinical Microbiology
2014-07-25 • Journal of Clinical Microbiology
We compared Mycobacterium tuberculosis sputum culture recovery and contamination rates between Lowenstein-Jensen medium (LJ) containing the following decontaminants and LJ alone: (i) PAN...
Journal Article
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Research

SAMBA HIV semi-quantitative test, a new point-of-care viral load monitoring assay for resource-limited settings

Ritchie AV, Ushiro-Lumb I, Edemaga D, Joshi HA, De Ruiter A,  et al.
2014-07-16 • Journal of Clinical Microbiology
2014-07-16 • Journal of Clinical Microbiology
Routine viral load (VL) testing of HIV-infected individuals on antiretroviral therapy (ART) is used to monitor treatment efficacy. However, due to logistical challenges, implementation o...
Journal Article
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Research

Designing HIV Testing Algorithms Based on 2015 WHO Guidelines Using Data from Six Sites in sub-Saharan Africa

Kosack CS, Shanks L, Beelaert G, Benson TT, Savane A,  et al.
2017-07-26 • Journal of Clinical Microbiology
2017-07-26 • Journal of Clinical Microbiology
Our objective was to evaluate the performance of HIV testing algorithms based on WHO recommendations, using data from specimens collected at six HIV testing and counselling sites in sub-...
Journal Article
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Research

Implementation of the thin layer agar for the diagnosis of smear-negative pulmonary tuberculosis in a high HIV prevalence setting in Homa Bay, Kenya

Martin AIC, Munga Waweru P, Babu Okatch F, Amondi Ouma N, Bonte L,  et al.
2009-06-03 • Journal of Clinical Microbiology
2009-06-03 • Journal of Clinical Microbiology
The objective of this study was to evaluate the performance of a low-cost method, the Thin Layer Agar (TLA), for the diagnosis of smear-negative patients. This prospective study was perf...
Journal Article
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Research

Whole genome sequencing has the potential to improve treatment for rifampicin-resistant tuberculosis in high burden settings: a retrospective cohort study

Cox HS, Goig GA, Salaam-Dreyer Z, Dippenaar A, Reuter A,  et al.
2022-02-16 • Journal of Clinical Microbiology
2022-02-16 • Journal of Clinical Microbiology
BACKGROUND
Treatment of multidrug-resistant or rifampicin-resistant tuberculosis (MDR/RR-TB), although improved in recent years with shorter, more tolerable regimens, remains largely...
Journal Article
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Research

Evaluating ten commercially-available SARS-CoV-2 rapid serological tests using the STARD (Standards for Reporting of Diagnostic Accuracy Studies) method

Dortet L, Ronat JB, Vauloup-Fellous C, Langendorf C, Mendeis DA,  et al.
2020-11-25 • Journal of Clinical Microbiology
2020-11-25 • Journal of Clinical Microbiology
Numerous SARS-CoV-2 rapid serological tests have been developed, but their accuracy has usually been assessed using very few samples, and rigorous comparisons between these tests are sca...
Journal Article
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Research

Detection of Plasmodium species by high resolution melt analysis of DNA from blood smears acquired in Southwestern Uganda

Kassaza K, Operario DJ, Nyehangane D, Coffey KC, Namugosa M,  et al.
2017-10-18 • Journal of Clinical Microbiology
2017-10-18 • Journal of Clinical Microbiology
ABSTRACT

Microscopic diagnosis of malaria using Giemsa-stained blood smears is the standard of care in resource-limited se...
Journal Article
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Research

Implementation of the thin layer agar method for diagnosis of smear-negative pulmonary tuberculosis in a setting with a high prevalence of human immunodeficiency virus infection in Homa Bay, Kenya

Martin AIC, Munga Waweru P, Babu Okatch F, Amondi Ouma N, Bonte L,  et al.
2020-12-21 • Journal of Clinical Microbiology
2020-12-21 • Journal of Clinical Microbiology
The objective of this study was to evaluate the performance of a low-cost method, the thin layer agar (TLA) method, for the diagnosis of smear-negative patients. This prospective study w...
Journal Article
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Research

Diagnostic accuracy of two rK39 antigen-based dipsticks and the formol gel test for rapid diagnosis of visceral leishmaniasis in northeastern Uganda

Chappuis F, Mueller YK, Nguimfack A, Rwakimari JB, Couffignal S,  et al.
2005-12-01 • Journal of Clinical Microbiology
2005-12-01 • Journal of Clinical Microbiology
The development of an accurate, practical, and affordable diagnostic test is essential to improve the management of visceral leishmaniasis (VL) in remote health centers. We evaluated the...
Journal Article
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Research

Use of Colorimetric Culture Methods for Detection of Mycobacterium Tuberculosis Complex Isolates from Sputum Samples in Resource-Limited Settings

Boum Y II, Orikiriza P, Rojas-Ponce G, Riera-Montes M, Atwine D,  et al.
2013-05-08 • Journal of Clinical Microbiology
2013-05-08 • Journal of Clinical Microbiology
Despite recent advances, tuberculosis (TB) diagnosis remains imperfect in resource-limited settings due to its complexity and costs, poor sensitivity of available tests, or long times to...
Journal Article
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Research

Prospective evaluation of the diagnostic accuracy of dried blood spots from finger-prick for the determination of HIV-1 viral load with the NucliSENS Easy-Q HIV-1 v2.0 in Malawi

Farjardo E, Metcalf CJ, Chaillet P, Aleixo L, Pannus P,  et al.
2014-05-01 • Journal of Clinical Microbiology
2014-05-01 • Journal of Clinical Microbiology
HIV-1 viral load (VL) testing is not widely available in resource-limited settings. Use of finger-prick dried blood spot (FP-DBS) samples could remove barriers related to sample collecti...
Journal Article
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Research

Copan eNAT Transport System to Address Challenges in COVID-19 Diagnostics in Regions with Limited Testing Access

Richard-Greenblatt M, Comar CE, Flevaud L, Berti M, Harris RM,  et al.
2021-04-20 • Journal of Clinical Microbiology
2021-04-20 • Journal of Clinical Microbiology
Community-based healthcare clinics and hospital outreach services have the potential to expand coronavirus disease 2019 (COVID-19) diagnostics to rural areas. However, reduced specimen s...
Journal Article
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Research

Use of filter paper as a transport medium for laboratory diagnosis of cholera under field conditions

Page AL, Alberti KP, Guenole A, Mondongue V, Lonlas Mayele S,  et al.
2011-06-22 • Journal of Clinical Microbiology
2011-06-22 • Journal of Clinical Microbiology
Confirmation of a cholera epidemic is based on bacteriological identification of the agent and requires the sending of samples to a culture laboratory, often in countries with limited re...